Publications

Latest Publications

Truncation-Free Genetic Code Expansion with Tetrazine Amino Acids for Quantitative Protein Ligations.

Eddins AJ, Bednar RM, Jana S, Pung AH, Mbengi L, Meyer K, Perona JJ, Cooley RB, Karplus PA, Mehl RA. Bioconjug Chem, 2023.

Ultra-Fast Bioorthogonal Spin-Labeling and Distance Measurements in Mammalian Cells Using Small, Genetically Encoded Tetrazine Amino Acids.

Jana S, Evans EGB, Jang HS, Zhang S, Zhang H, Rajca A, Gordon SE, Zagotta WN, Stoll S, Mehl RA. J Am Chem Soc, 2023.

Autonomous Synthesis of Functional, Permanently Phosphorylated Proteins for Defining the Interactome of Monomeric 14-3-3.

Zhu P, Stanisheuski S, Franklin R, Vogel A, Vesely CH, Reardon P, Sluchanko NN, Beckman JS, Karplus PA, Mehl RA, Cooley RB. ACS Cent Sci, 2023.

Site-specific dual encoding and labeling of proteins via genetic code expansion.

Bednar RM, Karplus PA, and Mehl RA. Cell Chem Bio, 2023.

Genetic encoding of 3-nitro-tyrosine reveals the impacts of 14-3-3 nitration on client binding and dephosphorylation.

Zhu P, Nguyen KT, Estelle AB, Sluchanko NN, Mehl RA, Cooley RB. Protein Science, 2023.

Tuning phenylalanine fluorination to assess aromatic contributions to protein function and stability in cells.

Galles GD, Infield DT, Clark CJ, Hemshorn ML, Manikandan S, Fazan F, Rasouli A, Tajkhorshid E, Galpin JD, Cooley RB, Mehl RA. Nature Communications, 2023.

High-yield recombinant bacterial expression of ¹³C-, ¹⁵N-labeled, serine-16 phosphorylated, murine amelogenin using a modified third generation genetic code expansion protocol.

Buchko GW, Zhou M, Hoang Vesely C, Tao J, Shaw WJ, Mehl RA, Cooley RB. Protein Science, 2023.

Structures of Methanomethylophilus alvus Pyrrolysine tRNA-Synthetases Support the Need for De Novo Selections When Altering the Substrate Specificity

Gottfried-Lee I, Perona JJ, Karplus PA, Mehl RA, Cooley RB. ACS Chem Biol, 2022.

Generating Efficient Methanomethylophilus alvus Pyrrolysyl-tRNA Synthetases for Structurally Diverse Non-Canonical Amino Acids

Avila-Crump S, Hemshorn ML, Jones CM, Mbengi L, Meyer K, Griffis JA, Jana S, Petrina GE, Pagar VV, Karplus PA, Petersson EJ, Perona JJ, Mehl RA, Cooley RB. ACS Chem Biol, 2022.

Accessing isotopically labeled proteins containing genetically encoded phosphoserine for NMR with optimized expression conditions

Vesely CH, Reardon PN, Yu Z, Barbar E, Mehl RA, Cooley RB. JBC, 2022.

Nanobody assemblies with fully flexible topology enabled by genetically encoded tetrazine amino acids.

Van Fossen EM, Bednar RM, Jana S, Franklin R, Beckman J, Karplus PA, Mehl RA. Sci Adv, 2022.

Creating a Selective Nanobody Against 3-Nitrotyrosine Containing Proteins.

Van Fossen EM, Grutzius S, Ruby CE, Mourich DV, Cebra C, Bracha S, Karplus PA, Cooley RB, Mehl RA. Front Chem, 2022.

Rational design for high bioorthogonal flurorgenicity of tetrazine-encoded green fluorescent proteins.

Tang L, Bednar RM, Rozanov ND, Hemshorn ML, Mehl RA, Fang C. Natural Sciences, 2022.